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Until the time of this study, thiamine suspensions had been prepared at the authors’ institution in a methylcellulose vehicle. This product had an expiration date of only 8 days when kept refrigerated. To the authors’ knowledge, there are no published stability studies for thiamine suspensions prepared in a 1:1 mixture of Ora-Sweet and Ora-Plus. The [...]

Regression analysis of the peak area ratio of thiamine to internal standard versus concentration demonstrated linearity over the working range of the concentrations, with coefficients of determination (r2) greater than 0.997 (n = 4). The intraday (n = 4) and interday (n = 4) coefficients of variation for the 3 different concentrations were within acceptable [...]

Degradation of Thiamine Thiamine 1.0 mg/mL was incubated overnight (about 16 h) at 100°C, made basic (to pH 12.89) with sodium hydroxide 10N solution 30% w/v (Fisher Scientific, lot SC9105042). The preparation was brought back to pH 3.9 with hydrochloric acid (Fisher Scientific, lot 29067), adjusted to a concentration of 0.3 mg/mL, and filtered. The [...]

Preparation of Stock and Standards Stock solutions of thiamine at 0.50, 1.00, 2.00, 3.00, and 4.00 mg/mL were prepared by diluting thiamine 100 mg/mL in HPLC-grade water (Fisher Scientific, Richmond, British Columbia, lot 034609). The internal standard was vitamin E (Aquasol E, Novartis Consumer Health Canada, Mississauga, Ontario, lot 1C046A) at a concentration of 10.00 [...]

Preparation and Evaluation of Suspensions Thiamine suspensions (100 mg/mL) were prepared from commercially available thiamine powder (Xenex Laboratories Inc, Coquitlam, British Columbia, lot 17695), in a 1:1 mixture of Ora-Sweet and Ora-Plus (Paddock Laboratories Inc, lots 2336330 and 2296122, respectively). Six replicates were pre­pared in separate 50-mL amber plastic prescription bottles; 3 bottles were stored [...]

INTRODUCTION Pharmacists prepare “homemade” suspensions for many medications that are not available in dosage forms suitable for children (and adults) who are unable to swallow tablets or capsules. Thiamine (vitamin B1), used in the treatment of metabolic diseases, is one such extemporaneously compounded suspension. Ora-Sweet (a sweetening agent) and Ora-Plus (a suspending agent) are now [...]