Archive for the 'Early Pregnancy' Category

23 Dec

Endometrial Responsiveness to Oxytocin: MATERIALS AND METHODS(5)

Isolation and Quantification of OTr mRNA Total RNA was isolated from 1 g endometrium by the guanidinium thiocyanate-phenol-chloroform extraction method. Each sample of RNA was treated with DNase I (Gibco BRL, Grand Island, NY), and purity of RNA was determined spectrophotometrically from the ratio of absorbance at 260 nm to that at 280 nm. Total […]

22 Dec

Endometrial Responsiveness to Oxytocin: MATERIALS AND METHODS(4)

RIA of PGF2a Concentrations of PGF2a in 25 (xl KRB were quantified using PGF2a (s.a. 195 Ci/mmol; New England Nuclear), antiserum to PGF2ci (1:24,150 final dilution), and Lutalyse (Upjohn Co., Kalamazoo, MI) for PGF2o standards as previously described. Separation of free and antibody-bound PGF2ct was achieved by incubation with 0.5% Norit A charcoal (JT Baker, […]

21 Dec

Endometrial Responsiveness to Oxytocin: MATERIALS AND METHODS(3)

Determination of PI Hydrolysis Hydrolysis of PI from endometrial explants was determined as previously described. Briefly, endometrial tissue from each gilt was dissected into pieces weighing 510 mg, and 100 ± 5 mg of tissue was placed into each of 6 vials. Ten microcuries of [3H]inositol (s.a. 18.8 Ci/mmol; Amersham, Arlington Heights, IL) was added […]

20 Dec

Endometrial Responsiveness to Oxytocin: MATERIALS AND METHODS(2)

Experiment 2 Endometrium was obtained from 30 gilts on Days 0, 5, 10, 12, 15, and 18 of the estrous cycle and Days 10, 12, 15, and 18 of pregnancy (n = 3/reproductive status X day group). The uterine horns were placed on ice immediately after removal at hysterectomy. Strips of endometrium (5 cm) were […]

19 Dec

Endometrial Responsiveness to Oxytocin: MATERIALS AND METHODS(1)

Animals Crossbred gilts, 5-6 mo of age, were observed daily for standing estrous behavior in the presence of an intact boar. Onset of estrus, after the occurrence of at least one normal estrous cycle (i.e., 18-24 days), was designated Day 0, and gilts were randomly allotted to cyclic or pregnant reproductive statuses in two experiments […]

18 Dec

Endometrial Responsiveness to Oxytocin during Diestrus and Early Pregnancy in Pigs(2)

Endometrial sensitivity to ОТ is markedly reduced during early pregnancy in sheep, cattle, and pigs. In pregnant ruminants, this reduced sensitivity is controlled through inhibition of OTr expression. However, it is unknown whether control of endometrial sensitivity to ОТ in swine occurs at the level of the OTr or via a post-receptor mechanism. buy antibiotics […]

17 Dec

Endometrial Responsiveness to Oxytocin during Diestrus and Early Pregnancy in Pigs(1)

Sensitivity to oxytocin (ОТ) in a variety of target tissues is widely regarded to be controlled through changes in ОТ receptor (OTr) population density resulting from corresponding regulation of OTr gene transcription. The ontogeny of endometrial expression of OTr in sheep and cattle is associated with development of uterine responsiveness to ОТ, resulting in OT-induced […]

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