Archive for the 'Lung Damage' Category

09 Mar

Upregulation of Collagen Messenger RNA Expression Occurs Immediately After Lung Damage: Conclusion

Following CPB, none of these patients developed ARDS. A possible explanation could be that, following activation, fibroblasts might display a form of programmed death signalized by negative growth regulatory peptides Another explanation could be that the initial extent of the host defense response is not sufficient to sustain the evolution of ARDS. A putative effector […]

07 Mar

Upregulation of Collagen Messenger RNA Expression Occurs Immediately After Lung Damage: Discussion

After CPB, collagen type I mRNA was significantly increased, with a mean rise of 92% above the pre-CPB period, over a period as short as 108 min. After a delay of 2 h, an increase of type I collagen protein would be improbable. However, since the level of collagen accumulation in the lung appears to […]

05 Mar

Upregulation of Collagen Messenger RNA Expression Occurs Immediately After Lung Damage: Histologic Analysis

Histologic Analysis n seven patients, the specimens obtained were large enough to allow biochemical and histologic analyses. The lung parenchyma in pre-CPB specimens was normal in all seven cases. The post-CPB specimens disclosed edema of the alveolar septa, the perivascular space, and the interlobular connective tissue. In addition, collapsed areas and interstitial infiltration with PMNs […]

03 Mar

Upregulation of Collagen Messenger RNA Expression Occurs Immediately After Lung Damage: RNA Extraction and Analysis

RNA Extraction and Analysis Tissue specimens were pulverized under liquid nitrogen. The tissue powder was homogenized in guanidine isothiocyanate (4 mol/L) guanidine isothiocyanate, 25 mM sodium citrate, pH 7.0, 0.5% sarkosyl, and 100 mM |3-mercaptoethanol) and phenol/ chloroform extracted. Quality of RNA preparations was examined by the integrity of 28 and 18S ribosomal bands in […]

01 Mar

Upregulation of Collagen Messenger RNA Expression Occurs Immediately After Lung Damage: Materials and Methods

Patient Selection The study was approved by the hospital’s ethical committee. All patients gave an informed consent before participating. Inclusion criteria were age younger than 70 years, no prior thoracotomy, absence of previous lung disease, and indication for mammary anastomosis (and therefore pleura opening during revascularization) for treatment of coronary arteiy disease. Surgical Procedure Patients […]

27 Feb

Upregulation of Collagen Messenger RNA Expression Occurs Immediately After Lung Damage

Despite advances in the critical care of ARDS patients, mortality is still high, exceeding 50%. The cause of death in such cases has been increasingly recognized as fibrosis, in association with irreversible respiratory failure, rising to 40% of the patients. While ventilator parameters and patient oxygenation indexes were not related to death, pulmonary biopsy findings […]

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