Archive for the 'Luteinizing Hormon' Category

20 Feb

Release of Luteinizing Hormone-Releasing Hormone: DISCUSSION(5)

GT1 cells cultured on beads required two days of culture and fetal hypothalamic neurons required three days of culture before pulsatile secretion of LHRH was detectable. This is in stark contrast to the present study, in which episodic LHRH release from enzymatically dispersed hypothalamic neurons from adult rats was detectable within 1 h of culture. […]

19 Feb

Release of Luteinizing Hormone-Releasing Hormone: DISCUSSION(4)

The primary hypothalamic cell culture perifusion system used in the present study provides a simple but elegant window through which researchers can evaluate the role of complex synaptic networks in the regulation of LHRH release. By comparing LHRH release from intact hemihy-pothalami with the release from the corresponding hemi-hypothalami that were enzymatically dispersed into largely […]

18 Feb

Release of Luteinizing Hormone-Releasing Hormone: DISCUSSION(3)

A recent innovative application of the in vitro perifusion technique used an immortalized LHRH neuronal cell line developed by Mellon and colleagues in a perifusion culture system. Use of the GT1 cell line has resulted in studies of second messenger systems associated with LHRH release and of cell-cell communication between immortalized LHRH cells. This model […]

17 Feb

Release of Luteinizing Hormone-Releasing Hormone: DISCUSSION(2)

The physiological importance of these observations may be subtle, since careful microscopic analysis of apposed hypothalamic cells im-munopositive for LHRH indicates that only ~5% of LHRH neurons in female rats and ~8% of LHRH neurons in the female monkey are connected. This is not to say that either direct synaptic connections between LHRH neurons or […]

16 Feb

Release of Luteinizing Hormone-Releasing Hormone: DISCUSSION(1)

The results of the present study provide compelling evidence in support of the hypothesis that coordinated LHRH release can occur in the absence of endogenous cytoarchi-tecture. Whether intact hemihypothalami or enzymatically dispersed hypothalamus tissue was perifused, LHRH release was episodic, indicating preservation of adequate cellcell communication to coordinate LHRH release from syn-aptically estranged neurons. A […]

15 Feb

Release of Luteinizing Hormone-Releasing Hormone: RESULTS

The LHRH secretion patterns of enzymatically dispersed LHRH neurons isolated from rat hypothalami in a perifu-sion culture are shown in Figure 1. LHRH release from this cellular preparation was pulsatile, as determined by analysis of the assay results using the PULSAR algorithm. The LHRH pulse frequency for enzymatically dispersed cells was 0.87 ± 0.15 pulses/h […]

14 Feb

Release of Luteinizing Hormone-Releasing Hormone: MATERIALS AND METHODS(3)

The system was run for ~10 h/experiment, and 1-ml fractions were collected at 10-min intervals using a refrigerated Gilson (Middleton, WI) FC205 fraction collector. Some of the tissues were challenged with 56 mM KCl 1 h before the completion of the experiment. Samples were frozen at -70°C until RIA. buy yasmin online LHRH RIA The […]

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