Archive for the 'Perfluorooctyl Bromide' Category

18 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Conclusion

The antioxidant NDGA is able to block directly and rapidly both the lipoxygenase and cyclooxygen-ase pathways, and NDGA suppressed mucin secretion both immediately (at 40 min) and after 4 h. Methylprednisolone only appeared to exert an effect after 4 h of incubation and did not show any effect at 40 min. Physiologically, methylprednisolone has a […]

16 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Inhibition of the secretagogue

The experiments allowing the perflubron to evaporate from the trachea after a 4-h immersion time were designed to mimic the clinical conditions that exist when PLV with perflubron is terminated and the fluorocarbon is allowed to evaporate from the airway surface during the switch to conventional mechanical ventilation. The data reported here are consistent with […]

14 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Discussion

We have previously demonstrated that surface tension-lowering agents (surfactants) can markedly increase mucociliary transport.- Because per-flubron has a lower surface tension than secretions, we hypothesized that there may be an increase in MCTR with perflubron exposure. Although a small increase in MCTR was noted, this was no greater than the increase seen with MARS. It […]

12 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Results

Evaluation of Secretagogue Potential The measured values of mucin secretion fell within the range detected by the standard curve. There was an 86.4% increase in mucin secretion over control from ferret trachea incubated for 40 min in perflubron (p = 0.006), a 17.6% increase after 4 h of exposure to perflubron (p = 0.02), and […]

10 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Data Analysis

Lysozyme Analysis by Spectrophotometry: Lysozyme is a bacteriolytic enzyme found in airway fluid, and the only airway source of this enzyme is the serous cells of the submucous glands. Lysozyme activity was measured spectrophotometrically by measuring the rate of lysis of a 1.38 mg/mL Micrococcus lysodeikticus suspension. An 0.1 mL volume of sample buffer was added to 1.4 […]

08 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Evaluation of Secretory Pathways

Evaluation of Secretory Pathways: The above protocol was also used to evaluate the physiological mechanism of mucin secretion with perflubron exposure, but tracheas were pretreated with specific inhibitors of stimulated secretion. After a 2-h recovery period, ferret tracheal segments were incubated for 40 min with each inhibitor in KHS or with KHS alone (period 1), then […]

06 Aug

Perfluorooctyl Bromide (Perflubron) Stimulates Mucin Secretion in the Ferret Trachea: Methods

Evaluation of Secretagogue Potential: Adult ferrets (weight, 1.7 to 2.4 kg; random sex) obtained from Marshall Farms (North Rose, NY) were killed by an intraperitoneal injection of pentobarbital sodium, and the trachea from larynx to carina was immediately removed. Each trachea was divided into four roughly equal segments from the upper trachea to the carina. […]

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