Archive for the 'Prostaglandin' Category

16 Jan

Regulation of Anion Secretion by Prostaglandin E2: DISCUSSION(3)

Further studies using specific EP receptor subtype agonists and antagonists are required to identify the receptor subtypes involved. The present finding that PGE2, when applied to either apical or basolateral membrane of the endometrial epithelium, gives rise to differential Isc responses (e.g., different potency and HC03 dependence) suggests the importance of PGE2 of different origins. […]

15 Jan

Regulation of Anion Secretion by Prostaglandin E2: DISCUSSION(2)

The present study also demonstrated the involvement of cAMP in the PGE2-stimulated Isc response. This hypothesis is supported by the mimicking of PGE2-stimulated Isc response by the cAMP-elevating agents forskolin and IBMX. The effects of forskolin, IBMX, and PGE2 are similar in that they all induce a sustained rise in Isc as previously reported for […]

14 Jan

Regulation of Anion Secretion by Prostaglandin E2: DISCUSSION(1)

Although PGs have been shown to play a versatile role in female reproductive physiology, their effects on electrolyte transport across the endometrial epithelium are less well known. Results of present study indicate that PGE2 induces anion secretion across the mouse endometrial epithelium. This hypothesis is supported by the effect of anion replacement, of both Cl […]

13 Jan

Regulation of Anion Secretion by Prostaglandin E2: RESULTS(3)

Involvement of cAMP in the PGE2-lnduced lsc Responses The involvement of cAMP in mediating the PGE2 responses was further tested using agents affecting intracellular с AMP level (Fig. 6). Additions of forskolin (10 |xM, n = 10), an adenylate cyclase activator, or IBMX (0.1 mM, n = 4), a cAMP-phosphodiesterase inhibitor, produced a sustained increase […]

12 Jan

Regulation of Anion Secretion by Prostaglandin E2: RESULTS(2)

Another set of experiments was performed to examine the sensitivity of the PGE2-stimulated Isc to various channel blockers. As shown in Figure 4A, amiloride, at a concentration (10 |xM, apical) known to block Na+ channels, reduced 70% of the basal current when added prior to the apical/basolateral addition of PGE2. However, it did not affect […]

11 Jan

Regulation of Anion Secretion by Prostaglandin E2: RESULTS(1)

lsc Responses to PGE2 The mouse endometrial culture exhibited a mean basal Isc (Ib) °f 6.2 ± 0.2 (xAcm 2 (n = 145), a mean transepithelial resistance (Rj) of 580 ± 20 Hem2 (n = 145), and a mean transepithelial potential (Vx) of 4.1 ± 0.2 mV (n = 145). The cultured mouse endometrial epithelium […]

10 Jan

Regulation of Anion Secretion by Prostaglandin E2: MATERIALS AND METHODS(3)

The measurement of Isc has been described previously. Monolayers grown on permeable supports were clamped vertically between two halves of the Ussing chamber. Monolayers were bathed on both sides with Krebs-Henseleit (K-H) solution, which was maintained at 37°C by means of a water jacket enclosing the reservoir. K-H solution had the following composition; NaCl, 117 […]

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