Archive for the 'Ribonucleic Acid' Category

29 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: DISCUSSION(4)

The corpus luteum and luteal cells respond to endotoxin and to acute inflammatory cytokines with a selective increase in Mn-SOD mRNA levels. This result is in good agreement with the previous reports showing that LPS, IL-1a, IL-1p, IL-6, and TNFa induce Mn-SOD expression in normal cells and in various cell lines. The cytokine-induced increase in […]

28 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: DISCUSSION(3)

Sugino et al. showed that the enzyme activity of Mn-SOD gradually decreased in the corpus lu-teum during the regression phase, although mRNA levels of Mn-SOD did not decrease in the present study. The increased mRNA levels and the decreased enzyme activity of Mn-SOD may indicate the enhancement of superoxide radical-associated metabolic activity. Interestingly, this finding […]

27 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: DISCUSSION(2)

The present study indicates that LPS injection decreases the serum progesterone level, suggesting that an inflammatory reaction may inhibit luteal function. LPS may stimulate macrophages to secrete cytokines, which in turn inhibit ovarian function. There is, however, evidence to suggest a direct effect of LPS. Since inflammation and cytokines cause the generation of superoxide radicals, […]

26 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: DISCUSSION(1)

To our knowledge, this is the first report showing the changes in luteal mRNA levels of Cu,Zn-SOD and Mn-SOD throughout pregnancy and the different regulation of both SODs by cytokines in the rat corpus luteum. Whereas Cu,Zn-SOD mRNA levels in the corpus luteum decreased and remained at low levels during the regression phase, Mn-SOD mRNA […]

25 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: RESULTS(3)

Similar dose-related up-regulation of Mn-SOD was obtained with IL-1a (Fig. 6), IL-1p (Fig. 7) and TNFa (Fig. 8). IL-6, however, was less potent at up-regulating Mn-SOD mRNA (Fig. 9A), and an increase in mRNA levels was observed at the highest dose only. These findings in GG-CL cells were similar to those in the corpus luteum […]

24 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: RESULTS(2)

To further examine the effects of LPS on luteal SOD expressions, whole corpora lutea from Day 15 of pregnancy were incubated with either LPS or LPS-induced inflammatory cytokines IL-1a, IL-1p, IL-6, or TNFa for 4 h in the absence of serum. All treatments induced an increase in Mn-SOD mRNA levels (Fig. 4A), but had no […]

23 Jan

Differential Regulation of Copper-Zinc Superoxide Dismutase: RESULTS(1)

In order to determine whether Cu,Zn-SOD mRNA and Mn-SOD mRNA are expressed in the rat corpus luteum, we performed RT-PCR to specifically detect Cu,Zn-SOD and Mn-SOD transcripts. As shown in Figure 1, a linear RT-PCR amplification of Cu,Zn-SOD, Mn-SOD, and S16┬ámRNAs was obtained using up to 25 cycles of PCR and 2 of total RNA. […]

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