Archive for the 'Sepsis' Category

30 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: Conclusion

Another finding of this study is the marked increase of IL-6 and IL-8 levels in BALF supernatant and the serum from patients with ARDS as compared with the control and the LTV group. These findings are compatible with previous reports. In recent studies, high levels of IL-8 were detected in the BALF of patients with […]

28 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: Discussion

Using an immunofluorescent technique, our study identified the strong expression of iNOS in AMs of human lungs predominantly at the early stage of ARDS following sepsis. No expression of iNOS was observed in the AMs from the control group, or the LTV group. Although the patients in both the ARDS group and the LTV group were […]

26 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: Results

Immunohistochemistry of iNOS and Cytokines of AMs Table 2 showed the results of the cytofluorometiy. The expression of iNOS was marked in seven of eight patients of the ARDS group and only one patient in the LTV group showed slight expression of iNOS. The same tendency was observed in IL-6 and IL-8 expressions. No significant […]

24 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: C ytofluorometry

The relative fluorescent intensity of immunostained AMs was measured semiquantitatively using an autostage cytofluorometer system (Nikon PI Auto-2; Nikon Inc; Tokyo, Japan) with a filter combination for FITC. The measurements were done by the pathologist who was blinded to the diagnosis of the patient. The system is composed of a compact epi-illumination cytofluorometer equipped with […]

22 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: Radioimmunoassay of IL-lp

IL-1(3 concentrations in the serum and BALF were determined by radioimmunoassay (RIA) using an IL-1(3 (I) RIA kit (Medgenix Diagnostics SA; Fleurus, Belgium) as previously reported. The detection limit of the assay was 10 pg/mL, and the interassay coefficients of variation are 2% and 3.2%, respectively. Enzyme-Linked Immunosorbent Assay of IL-6 and IL-8 The human […]

20 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: Bronchoalveolar Lavage

BALF from the ARDS patients was obtained within 24 h after ARDS was diagnosed. BALF from the LTV group was obtained 1 week after endotracheal intubation. Blood sampling was performed concurrently. All patients were sedated and preoxygenated (FIo2=1.0). A flexible bronchoscope (type P10; Olympus Corp of America; New Hyde Park, NY) was inserted to obtain […]

18 May

Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages of ARDS Following Sepsis: Materials and Methods

Study Population Patients with ARDS were identified prospectively. ARDS was defined according to the definition of Murray et al as follows: (1) lung injury score >2.5; (2) wedge pressure <18 mm Hg measured by pulmonary catheter, or no clinical evidence of congestive heart failure; and (3) no other explanation for the above findings. Eight patients […]

Pages: 1 2 Next

© 2008 HIV/AIDS News & Information